Journal: International Journal of Molecular Sciences

Article Title: PPAR Alpha Activation by Clofibrate Alleviates Ischemia/Reperfusion Injury in Metabolic Syndrome Rats by Decreasing Cardiac Inflammation and Remodeling and by Regulating the Atrial Natriuretic Peptide Compensatory Response

doi: 10.3390/ijms24065321

Figure Lengend Snippet: Effect of treatment with clofibrate on infiltrated cells and collagen volume fraction (CVF) in hearts with ischemic/reperfusion (I/R) damage. In ( A ), the sites where cellular infiltrates are located are indicated with arrows in images stained with hematoxylin–eosin (HE). In ( B ), the fluorescence areas with collagen deposits are distinguished in red with picrosirius red (PSR) staining. Representative images of histological examinations are presented and the mean ± SEM values of the number of infiltrated cells and the mean ± SEM of CVF % are shown in the graphs. a p < 0.0001 vs. Ct-Sh; b p < 0.0001 vs. Ct-Sh; c p < 0.0001 vs. Ct-V-I/R; f p < 0.0001 vs. MetS-Sh; g p < 0.0001 vs. MetS-V-I/R. Abbreviations: Ct = control; MetS = metabolic syndrome; Sh = sham-operated rats; V = vehicle; Clo = clofibrate. Bar = 100 μm.

Article Snippet: Myocardial collagen volume fraction (CVF) was measured by picrosirius red (PSR) staining (ab150681, Abcam PLC, Cambridge, UK) [ , ].

Techniques: Staining, Fluorescence